Formulas used in Our systems

Welcome to our dedicated formula resource page, where you’ll find detailed information on the formulas and calculations driving PhenoVation’s advanced camera systems. These systems are designed specifically for accurate multispectral imaging, photosynthesis imaging, and plant phenotyping, essential for research and development in plant science.

Formulas Photosynthesis Parameters

·        Fv/Fm   =  (Fm - F0) / Fm

·        Fq’/Fm’ =  (Fm’ - Fs’) / Fm’

·        ETR        =  Fq’/Fm’ * Abs * PSIIratio * Actinic (PSIIratio = 0.5)

·        NPQ      =  (Fm - Fm’) / Fm’

·        F0’         =  F0 / (Fv/Fm + F0/Fm’)

·        qP          =  (Fm’- Fs’) / (Fm’- F0’)

·        qL          =  qP * F0’ / Fs’

·        qN         =  1 – (Fm’- F0’) / (Fm – F0)

·        qE          =  Fm * (Fm’’ - Fm’) / (Fm’’ * Fm’)

·        qI           =  (Fm - Fm’’) / Fm’’

·        ɸNO      =  1 / (NPQ + 1 + qL * Fm/F0)

·        ɸNPQ   =  1 - Fq’ / Fm’ - ɸNO

·        NPQ(t)  =  4.88 / (Fm’/F0’ – 1) – 1

·        RFd        =  (FmRFd - FtRFd) / FtRFd

Filters used for Photosynthesis Measurement

PlantExplorer XS uses 600nm long pass filter

PlantExplorer PRO+ uses 730nm bandpass

PlantExplorer MAX+ uses 730nm bandpass

Formulas used for Spectral measurements

·        Abs    =  1 - Rred/Rfarred   

·        ChlIdx   =  (1/Rrededge - 1/Rnir) * Rnir

·        AnthIdx =  (1/Ranth - 1/Rrededge) * Rnir

·        NDVI = (Rnir- Rred)/(Rnir+Rred)

Filters used for Multispectral measurements

Rred = 640nm

RFarred = 732nm

Ranth filter = 512nm

Rrededge  filter = 710nm

Red = 640nm

Nir = 769nm

Formulas used for  Color Parameters

·        Normalise R,G,B values in a number between 0.0 and 1.0

·        Max and Min are the minimal and maximal of the (R, G, B) values

·        (H, S, V) are defined as:

·        If (R = Max)          H = (G - B) / (Max – Min) * 60

·        If (G = Max)         H = 2 + (B - R) / (Max – Min) * 60

·        If (B = Max)          H = 4 + (R - G) / (Max – Min) * 60

·        S = (Max - Min) / Max

·        V = Max


Filters used for Color measurements


Red = 640nm

Green= 550nm

Blue = 475nm

A man is smiling for the camera and wearing a black shirt.

Do you have a question?

We are here to answer all your questions. Don't hesitate to contact our experts to find out everything about photosynthesis, color imaging and everything in between.

Talk with us!

What do our clients say?

Our references.

We've had the privilege of working with PhenoVation for nearly a decade, and throughout this time, they have consistently proven to be a reliable and valuable partner. Their expertise, dedication, and innovative solutions have been crucial for our success, and we look forward to many more years of collaboration.

Dr. David Han (PhenoTrait)

Magdalena
PhenoVation’s attention to detail and commitment to excellence have been pivotal in creating our state-of-the-art phenotyping facility. Their involvement goes beyond supplying imaging equipment—they've shaped our entire system.

Magdalena Julkowska (Boyce Thompson Institute)

Reference from Bayer employee
Our collaboration with PhenoVation began over a decade ago with a project to integrate fluorescence sensor technology into a high-end phenotyping platform. This partnership has since grown into a strong and lasting relationship, marked by outstanding support and innovative contributions that have significantly advanced our plant physiology research.

Sina Roth (Bayer)

Quote from Kris Audenaert from Ghent University
Since 2017, we have relied on the Plant Explorer Pro for our scientific research and collaborations with industrial partners. The equipment’s reliability, robustness and high quality have been essential to our work. PhenoVation’s excellent service has ensured that we can always count on the multispectral imaging platform to meet our scientific and operational needs

Prof. Kris Audenaert (Ghent University)

In our 10 years working with PhenoVation, we have found their technology reliable, easy to use, and critical for answering our plant science research questions. The customer service and technical development are top-notch!

Dr. Katie Murphy (Donald Danforth Plant Science Center)

John Doe's Image
Over the years, PhenoVation has been a key partner for us in realizing high throughput automated phenotyping projects. As a system integrator, we team up with specialized companies of various disciplines, the expertise and service of PhenoVation has allowed us to realize beautiful projects.

Michael Meijler WPS

Quote from Noémie De Zutter from Ghent University
Our advanced phenotyping system ensures a smooth and efficient interaction between the sensor and the plants, delivering unique measurements with user-friendly software. The combination of chlorophyll fluorescence, spectral proxies and GFP-measurements provides highly valuable insights into plant-pathogen interactions. The team at PhenoVation is very approachable and involved, always ensuring a quick and reliable service

Dr. Noémie De Zutter (Ghent University)