Multiple pepper seeds germinating

20 pepper seeds are germinated on filter paper covered by a black membrane. During the germination color images (left side) and  chlorophyll fluorescence images are grabbed. The calculated efficiency of photosynthesis is also shown (right side). Before radicle protrusion the efficiency of photosynthesis is already increasing, indicating methabolic activity in the seed. The seeds which show the biggest increase of efficiency germinate first.

Pepper seed germination

During the germination phase the embryo of a pepper seed synthesizes chlorophyll. Since the seed coat is translucent, the metabolic activity can be monitored in time by using the CropReporter. This movie shows the increase of chlorophyll fluorescence (brighter color means higher fluorescence signal). Notice the increase of chlorophyll fluorescence starts way before radicle protrusion.

Pepper seeds normal and pre-sprouted

Soybean seeds and fluorescence

In this example two batches of soybean are being germinated. The left batch is of poor quality, contains a lot of so called ‘green seeds’, seeds which are not fully mature. Using the CropReporter technology the images show that these seeds have already in dry state a high chlorophyll fluorescence signal. The right batch is of high quality, these seeds have in dry state a low fluorescence signal. When the water uptake starts, the fluorescence signals of the seeds of high quality increases, indicating methabolic activity. At a certain level of fluorescence the seeds germinate. The colors used indicate the level of fluorescence signal, low to high: black-red-orange-yellow-green.